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To provide an overall appropriately balanced dataset, treatment group samples were distributed between array plates and hybridization batches.
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(A) PP/PN fold-change comparison (PP/PN) between GSE51440 (HT HG-U133+ PM array plates) and datasets generated using Affymetrix Human Genome U133 Plus 2.0 arrays.
Samples were then applied to the Rat Gene 1.1 ST 16 Array Plate or 24 Array Plate and placed in the GeneTitan System (Affymetrix), according manufacturer's recommendations.
The Axiom array plate and hybridization tray are then loaded onto the GeneTitan MC Instrument.
Likewise, cluster analysis did not identify outliers and suggested good agreement between HT HG-U133+ PM array plates (GSE51440) and the U133 Plus 2.0 platform (Additional file 1: part M).
b Black silicon nanowire array plate fabricated and used for the drying experiment.
MPGV, KHGK-J and PMW-K isolated DNA and prepared microtitre array plates for genotyping.
Printed array plates were sealed in airtight bags and stored at room temperature before use.
The ΔΔCT and fold-change values between the two RNA samples for each gene on the array plate were obtained and compared between the two sites and across different real-time PCR thermal cyclers.
Following manufacturer's instructions, we prepared labeled cDNA, trays, and arrays with the GeneTitan Hybridization, Wash and Stain Kit for WT array plates (Affymetrix).
RNA was extracted and hybridized to high-throughput GeneChip® Human Genome U133 Array Plates (Affymetrix).
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