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The SureSelect hybrid capture protocol is faster and easier to perform than the Nimblegen protocol, which is a general difference between array and solution based capture methods rather than between these two methods in particular.
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Recently, array and solution based hybridization techniques have been developed for target sequence enrichment [23] [25].
This is consistent with results from the same group investigating PDZ domain binding using a similar protein microarray method which concluded that the technique resulted in a false positive rate of approximately 50%, and poor correspondence between array-estimated and solution-binding measured equilibrium-dissociation values [ 60- 62].
Their model takes into account the relative lifetime differences between arrays and between the scalars covered by each array.
The SGH Array was tested for reproducibility both between arrays and within the same array.
A mixed-model approach was chosen to adequately describe between-array variation, and utilized the probe-replicates in each array.
Relationship between array names and pointers.
Relationship between array names and pointers 3-b.
Concordance between array CGH and SNP array in the detection of CNA was investigated.
Within-array and between-array normalization was performed using loess and scale standardization.
For within array and between array normalization, print tip Loess and scale was used.
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