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By analyzing the associations between array SNPs and deviations in BMD determined by DXA, we determined that a common variant in the 3′-flanking region of the GPR98 gene, rs10514346, is a candidate BMD-related polymorphism.
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The imputation accuracy improved non-linearly with increasing LD between the 90 k array SNPs and most closely linked 9 k array SNP for all four applied imputation algorithms irrespective of the MAF class.
Tiling array SNP analysis and ORF array CGH data were obtained as previously described [ 4].
After removing uninformative and poorly performing SNPs, these arrays contained 181,752 (A-array) and 180,976 (B-array) SNP assays, and the set of SNPs on each array did not overlap.
SNP intensities were extracted using the dChip software and subsequently all intensities were normalised to facilitate comparisons between SNPs and arrays, as described in Supplementary Material.
In total, there was a discrepancy in 19 (29%) samples between SNP array and IHC/FISH analysis.
Two high-density single nucleotide polymorphism (SNP) genotyping arrays have recently become available for bovine genomic analyses, the Illumina High-Density Bovine BeadChip Array (777,962 SNP) and the Affymetrix Axiom Genome-Wide BOS 1 Array (648,874 SNP).
We first determined PIGB expression levels in 37 LCLs selected on the basis of genotypes for those 3 SNPs using both QRT-PCR assay and expression array data to confirm the association between the SNPs and PIGB expression.
Covering genomes with high resolution, single nucleotide polymorphism (SNP) genotyping arrays facilitate the detection of associations between SNPs and phenotypes.
The discrepancy between SNP arrays and FISH can be mainly explained by the fact that 12 amplicons included the centromeric region of chromosome 17 that harbors the binding site of the CEP17 reference probe.
However, all these aforementioned methods fail to account for the spatial correlation between SNPs, and the correlation between adjunct SNPs could be as high as 0.99 for high density SNP array such as Affymetrix 500K.
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