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Between any two genotypes: Search can be executed for variations between any two genotypes of interest.
Genetic similarities between any two genotypes were estimated according to Nei and Li [ 78].
The genetic similarity between any two genotypes was estimated based on Jaccard's similarity coefficient.
Furthermore, mutations in regulatory regions are likely to result in differences in gene expression between any two genotypes.
In both analyses, binomial exact tests were used to identify statistically significant differences in expression between any two genotypes.
The different paths on the hypercube represent all the different trajectories between any two genotypes within this genotype space.
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DREB/CBF regulons are also equally functional even between any two rice genotypes that differ significantly in regard to relative stress tolerance.
Anxiety-like behaviors measured by open-field (as path in the center) and EPM (as time spent in the open arms), exploiting rodents natural aversion to open space, did not show any differences between the two genotypes (data not shown), leading to the conclusion that PTPH1 may not be involved in the integration of thalamo-limbic information, key paths for anxiety behavior processing.
Moreover, when we assessed immature granulocytes numbers by flow cytometry, we did not find any significant differences between the two genotypes (Supplemental Fig. S1).
Because the genomes of C418 and K354 are almost identical, any transcriptomic differences between the two genotypes must be due to the presence of fragments introgressed from Bg300, whether caused by direct allelic replacement or genome-wide transcriptome reconfiguration as a result of the replacement.
We obtained the expected results from 32 sets of primers between the two genotypes.
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