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Micronuclei formed after mitosis from lagging chromatids or chromatin bridges between anaphase chromosomes and were stably maintained in the cells for up to one cell cycle.
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The detached chromatid was located between the separating anaphase chromosomes and may be merotelically attached to the spindle [19].
Early micronuclei were derived from either the chromatin bridge between separating anaphase chromosomes (Fig. 3A) or the chromatid detached from the bulk of the chromosomes during the transition from metaphase to anaphase (Fig. 3B).
Contractile ring assembly begins immediately after anaphase chromosome segregation and requires the contractile ring localization of the small GTPase Rho.
It also induced anaphase chromosome movements and PBE with kinetics similar to wild-type.
The knock-down of CENPF with specific siRNA caused defects in metaphase chromosome alignment, anaphase chromosome segregation and cytokinesis [ 23].
During anaphase, chromosomes are separated by moving from the metaphase plate to the spindle pole (anaphase A) and by the elongation of the mitotic spindle (anaphase B).
Moreover, spindle pole clustering could be inhibited to uncouple spindle multipolarity from anaphase lagging chromosomes, and thus demonstrate the causal relationship between these two phenomena.
The anaphase chromosome compaction was perturbed and lagging chromosomes were observed (Fig. 3).
This recruitment plays critical roles in ensuring timely mitotic progression and anaphase chromosome movement.
The targeting of damage to anaphase chromosome tips likely includes telomeric regions, and suggests a specific cellular response when these regions are damaged during anaphase versus damage to other regions of the chromosome.
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