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The most significant change between P2a and P2e was the change in surface shape at site B, which was maintained between P2e and P2j, whereas site A appeared to vary substantially in shape between all three clusters (Figure 5).
The microsatellite genetic diversity analysis showed that pairwise differences and F ST were significant between all three clusters (Table 4), which was not always the case between animals from different SLs within each of the clusters (Table 5).
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One-way ANOVAs revealed a reliable ablation effect between the three clusters for all dependent variables in pallium and VZ/SVZ (F = 10.19 69.66; df = 2,34; Pα<0.01).
The genetic distance between these three clusters were approximately 85%.
Note the seven individuals with clear intermediate positions between the three clusters.
Multidirectional associations may exist between the three clusters of independent variables and the dependent variables.
Overall, the differences of scores between the three clusters implied support for moderate convergent validity.
Additionally, no overlap was detected in the enriched regulations (TFs) between the three clusters.
Statistical analysis provided the distinction between three Clusters reflecting different hydrochemical processes.
The pairwise distance of the VP1 amino acid sequence was calculated between all of the strains in three clusters, and a 5% amino acid difference cut-off was used to define GII.17 new variants (Supplementary Table S4).
It should be noted, however, that though fire risk perceptions were significantly different between the two clusters, all mean values were above four on a five-point scale.
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