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(E) Patient outcome between all stage groups was compared using a log-rank test.
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Transit times and speeds were compared graphically between clinical stage groups.
Wide overlap of transit times and transit speeds was observed between clinical stage groups.
For comparison of differential gene expression between different stage groups, the background was removed and data were normalized in accordance to the GC-RMA method [ 43].
The Kruskal-Wallis test was used to check for differences in number of altered chromosomes and number of mutations between TNM stage groups I-III and IV.
Heterogeneity between stage groups can be assessed by comparing stage-specific survival estimates for significant differences.
Wang et al. showed decreased heterogeneity between stage groups in the 7th edition as well.
Second, there should be discrimination between stage groups; patients in different stage groups should have larger differences in survival.
Whether differences between stage groups are significant is highly dependent on the size of the data set.
Small differences in survival estimates between stage groups are more likely to be statistically significant in a large data set.
Overall, in the AJCC 6th edition, two out of six consecutive steps between stage groups were not significantly discriminant, while in the AJCC 7th edition, three out of seven consecutive steps were not significantly discriminant, indicating that the discriminant value between stage groups has decreased between the 6th and 7th edition staging system.
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