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For the nuclear data set, there was a clear tradeoff between alignment length and taxonomic coverage.
Given the tradeoff between alignment length and taxonomic coverage, we compiled a dataset of 10502 nucleotide sites for 11 species in the Cracidae and 40 in the Galloanserae that have complete mt genomes.
However, we find no relationship between alignment length and evolutionary distance in either of the core sets (11-way core set: Radj = 0.05, P = 0.25; 15-way core set: Radj = 0.03, P = 0.26).
Very similar results were obtained with a 1,340-gene 1,340-geneic data matrix in which all genes had the same length, arguing that the well-known correlation between alignment length and phylogenomic signal datanot have a matrixinfluence on phylogenetic reconstruction in this lineage.
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Additional file 7: Correlation between alignment length, tree incongruence and bootstrap proportions.
We explored several classification thresholds, including alignment e-value, alignment percent identity, and the ratio between the alignment length and the read length (i.e., coverage).
The absence of a strong correlation between relative alignment length and hybridization ratio may be due to insertions in the heterologous sequence that result in reduced hybridization strength and inflated relative alignment length (even above 100%).
The best balance between expected alignment length and percent identity, which are calculated by the High-scoring Segment Pair (HSP) of the blast algorithm, was provided by the EST specific parameters [see Additional file 1, Table S1].
For example, referring to Tables 3 and 4, the seed alignment of RF00230 (RF00559 and RF00468, respectively) has a length of 262 nt (81 nt and 66 nt, respectively) and MCC value of 0.83 (0.91 and 0.65, respectively), showing no direct relationship between the alignment length and the MCC.
One group of the contigs shows a clear relation between alignment length of the contig to total contig length.
The latter may be due to differences in the examined alignment length and between local and global alignments.
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