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The first crystal structures of engineered FN3 molecules in complex with their targets have been solved, and structural studies of engineered FN3 have begun to reveal determinants of stability and to define zones that accept mutations with minimal trade-off between affinity and stability.
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Hereby, direct correlation between the affinity and stability of the RMSD graphs for GC rich sequences over AT rich ones was established.
The dimensionless parameter (i.e., ratio of binding affinity) introduced in this work from such experiments is useful in quantitatively depicting such binding affinity, indicating that the binding affinity and stability between AuNPs and catecholamine is approximately 1.5 times stronger than that between amine and AuNPs.
Makrijannis, A. & Deng, H. (Univ. Connecticut) Retro-anandamides, high affinity and stability cannabinoid receptor ligands.
Mchugh, L. et al. Increased Affinity and Stability of an Anti-HIV-1 Envelope Immunotoxin by Structure-based Mutagenesis *.
The ligands have to show high affinity and stability towards the metal ion in question and low affinity towards the stone material itself, mainly Ca2+.
The effects may be long lasting due to the high affinity and stability of the sulfated compound-virus complex, as evidenced by surface plasmon resonance studies.
Because of the low affinity, scFv IIB6 was affinity and stability matured.
Their multiligand chelation ability enhances their binding affinity and stability.
Oxygen binding encompasses structural changes at the interfaces between the α1β2 and α2β1 dimers, but also subunit interactions are important for the oxygen binding affinity and stability.
After comparing native reactivity, antibody affinity and stability, the best antibody-producing cell line was chosen.
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