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One may expect that similarity between a base sequence and a known binding motif is a necessary condition for binding, but it is neither a necessary, nor a sufficient one.
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The Genomic Assay® reported herein depends upon YOYO-1 bis-intercalation rendering the duplex target into a locally de-condensed conformation [23], which allows for specific base recognition and detectable binding to occur between a heteropolymeric base sequence of a strand in the duplex and the heteropolymeric base sequence of the probe strand.
The relationship between the base sequence and the amino-acid sequence constitutes the genetic code.
This potential suggests that by routinely applying the present analysis to different nucleic acid sequences undergoing various structural changes, it may be possible to achieve a detailed and robust understanding of the relationship between base composition, base sequence and structural heterogeneity in nucleic acids.
Discrimination is based on a kernel machine binary classifier [ 50] capable of computing similarity between base sequences in terms of their respective putative secondary structures [ 34] and thus of the spatial conformation of their binding sites (see Methods for a detailed description of the classifier).
Single-end, 100 base sequencing generated between 7 and 12 million reads per library.
The forward primers contained a 10 base barcode sequence positioned between the adapter and the primer sequence.
Errors were defined as base pair inconsistencies between an individual sequence and the most common base pair found in that position in the alignment of all sequences.
People find it easier to discuss the possible hazards of genetic testing and modified crops than the complicated mechanics that lie between a sequence of DNA bases and a physical trait in an organism.
To detect sequence similarity, most methods use BLAST, a fast heuristic algorithm for identifying homology between sequences based on local sequence alignments.
Robustness is measured using neutrality, which is calculated as the mean secondary structure similarity (i.e. normalized base-pair distance) between a sequence and those that differ by exactly one point mutation 1-mutantt neighbors) [ 29].
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