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LD metrics between SNPs were based on European samples (CEU) from HapMap release 28 [ 18].
Measurement of LD between SNPs were based on Data Release 27, phase 3 (Feb 2009 on NCBI B36 assembly, HapMap dbSNP126), viewed using Haploview software (v4.2) and plotted using SNP annotation and proxy search (SNAP).
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The greatest attrition of these candidate SNPs was based on SNP conservation between reads from a single germplasm, and 55% in silico SNPs were rejected [ 12].
SNPs positions were based on the UMB 3.1 bovine assembly.
Markers were based on SNPs (single nucleotide polymorphisms) identified between the two parents.
Two functions were used to model LD between two SNPs: one was based on distance [ 24] and one was based on difference in MAF [ 25].
As an index of the level of OR polymorphism, a mean distance N between SNP was calculated, based on the number of SNP detected through the pairwise comparison of all OR sequences and the occurrence of the two alleles of each SNP.
The greatest attrition in candidate SNPs, however, was based on SNP conservation between reads of a single germplasm (selecting 100% consistency within genotypes).
Significance of associations between SNPs and traits was based on threshold p < 2 × 10−6 (i.e. −log10(p) = 5.7), a stringent Bonferroni correction calculated by dividing 0.05 by the total number of SNPs in the analysis, 24 246.
Interaction between SNPs was analyzed using a model based on allele dosage.
SNP clustering was based on strain-strain pair-wise distances computed by counting the number of SNPs that differ between each of the strains divided by the total number of SNPs that are typed in both strains.
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