Suggestions(1)
Exact(6)
No comparisons can be made between CT values for the samples.
The association between Ct values for probe A and smear grade was summarized and tested based on Spearman's rank correlation.
Since gene expression was determined by only one sample (similar to gene expression studies by RT-PCR) no comparisons can be made between CT values for the samples.
GG2, genogroup 2. For samples that were reactive in both rectal swabs and faeces there was a significant correlation between Ct values for all agents (R 0.31-0.85, Figure 1).
The percentage of MYCT1 promoter that was bound with c-Myc B was calculated based on the differences between Ct values for input and DNA samples immunoprecipitated from Hep-2 cells treated or untreated with 5-zaz [ 25].
The percentage of HSD17B1 promoter that was bound with Pol II was calculated based on the differences between CT values for input and DNA samples from cells treated or untreated with 5-dAzaC.
Similar(54)
The relative gene expression level was determined by the ratio between Ct value for ICAM-1 and β-actin.
This is achieved by employing the equation (1) wherein ΔCt stands for the difference between Ct values of gene of interest and housekeeper β- Actin.
For each sample, the difference (dCt) was calculated between Ct values obtained for target and reference amplicons.
Differences between the Ct values for experimental and reference genes (18S rRNA) were calculated as ΔΔCt.
Differences between the Ct values for experimental and reference genes (18srRNA) were calculated as ΔΔCt. 1 ug of pcDNA3.1 containing the FMR4 sequence (or without the sequence as a control) was utilized for in vitro transcription using a MAXIscript kit (Cat # AM1200, Ambion, Austin, TX) according to manufacturer's instructions.
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