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Google Cloud's Text-to-Speech and Speech-to-Text APIs are getting a bunch of updates today that introduce support for more languages, make it easier to hear auto-generated voices on different speakers and that promise better transcripts thanks to improved tools for speaker recognition, among other things.
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Since better correlating transcripts between FFPE and FF matching samples are likely less affected by the degradation, we wanted to evaluate if a difference in detectability could be related to the biological function of the transcript.
While some of the newly developed tools may perform better building transcripts, a manually curated gene set is still highly recommended for troubleshooting, particularly for non-model organisms.
It has been reported that PCR amplification requires less RNA, is more reproducible and generates better target transcripts than linear amplification [ 5, 13], at least if the sequences are limited to the 3'- end.
There was relatively good coverage of reads aligning to DLD from QTC4 (a susceptible T. castaneum population, accession JX434604) in all four treatment groups (Additional file 5), although depth of coverage was better in transcripts from the resistant insects in some regions.
As shown in Additional file 9: Figure S4B, most of the volatiles remained associated with the same cluster members, as previously described in Figure 1 (clusters C2, C5, C6, C7, C8, C9, C10, and C11), while other metabolites (those belonging to clusters C1, C3, C4, C12, and C13) correlated better with transcripts as compared to other volatiles and formed new clusters.
As shown in Figure 2E, for each transcript a value was associated which represents how the transcript fits in the classes, the higher is the value the better this transcript is representative of this class.
To better quantify transcript abundances in An. quadriannulatus, a modified version of the An.
We also evaluated whether DNA methylation levels of a suite of mechanism-based candidates explains birth weight trait variance better than transcript level of the same genes.
We also located 45 potential pathologs not representing mouse orthologs of human genes, as there was a better mouse transcript match for the human gene they share sequence homology with.
II The phaseogram issued from De Risi's group was ordered according to every class of TAPs from I to IV and then compared to Winzeler's data that evaluate better the transcript levels, therefore leading to complementary information.
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