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Often, a better sampling may be obtained if a nonsymmetrical sampling function is employed.
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The sampling may not be representative and respondent-driven sampling may be a better design.
Though the sample size is large enough to compare the difference in the mRNA expression between the EAC and control, a large size of samples may be better to fully examine the mRNA expression of IGFs in the EAC.
In some cases, the caulking has clearly lost its elasticity and the extent of degradation in any caulking material sample may be a better predictor of the amount of PCBs that can be mobilized than the bulk PCBs content of the caulk.
For example, a wing may be removed and preserved separately as a spread wing for better study of flight feathers; a tissue sample may be removed and frozen for molecular analyses; or a recording of the bird's song before collection may be archived.
In most cases this difference is large (Additional file 7), hence, the mean RE-score in a sample may be a much better predictor of the expression level of the targets of any particular miRNA, than is the expression profile of the miRNA itself.
Although an internet sample may be biased towards the more wealthy and better educated, 80% of New Zealand households have internet access.
Random sampling methods work better than transect methods if there is heterogeneity, although larger numbers of samples may be required to detect rare species (Goslee 2006).
Positive control samples may be kept where the appropriate microbiological expertise and proper quarantine facilities are available; otherwise it is better to avoid keeping infected material.
Studies using better instruments can be performed with smaller sample sizes, but better instruments may be more expensive in terms of dollars, opportunity cost, or poorer data quality due to the response burden of longer instruments.
Still, somewhat better times may be ahead.
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