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To better quantitate the sensitivities of specific PrP epitopes to PK or trypsin cleavage, we measured protease-resistant epitopes by direct ELISA (Fig. 2A).
To better quantitate the inhibition of WT and mutant 3Dpols by the inhibitors we used a filter binding assay that can be run efficiently in a 96-well format.
To better quantitate these experiments, we performed colony assays.
To better quantitate β-synemin expression during muscle regeneration, immunoblot analysis was performed for muscles collected at different time points following cardiotoxin injection.
To better quantitate the extent of necrosis and apoptosis in the various conditions, we compared Annexin V and TOPRO labeling at several time points (Fig. 2c).
Tracer developments have been matched with advances in instrumentation and analysis methods to better quantitate and monitor disease in both patients and preclinical models.
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To better examine and quantitate the neutrophil potential of generating NET, we used flow cytometry to measure neutrophils containing citrullinated Histones.
This framework considers the dose response and variability associated with each key event to better understand and potentially quantitate the impact of each of these factors on the risk assessment as a whole.
Thus, multi-divergence point models may be better equipped to detect and quantitate temporary shifts in evolution.
Purified rPfGK was quantitated using Coomassie Plus-the Better Bradford™ Assay kit (Pierce , USA.
That process will take time, but perhaps a first step could be to improve our ability to accurately quantitate HER2 protein expression and try to gain a better understanding of how differences in expression levels among individual breast tumors affect the natural history of HER2-positive breast cancer and, more importantly, response to HER2-targeted agents like trastuzumab.
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