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Advances in molecular biology and protein biochemistry have led to the development of several modern technologies to better examine the expression, post-translational modification and functional alteration of proteins at single-protein and proteomic levels.
To better examine the expression levels in the context of the rest of the assembly, we added the F. heteroclitus cDNA sequences to the assembled contigs and recalculated differential expression for these in the context of the rest of the assembled sequences.
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To better examine the effect of FLCN expression on TFE3 post-translational modifications, FLCN and TFE3 were ectopically expressed in UOK257 cells using adenoviral vectors, and the cell lysates were separated by either 4 20% or 7.5% SDS PAGE (Fig. 3C).
To better examine the kinetics of recombinant protein expression from the TMV vector we prepared total soluble protein extracts from A. tumefaciens/pJL43 GFP + A. tumefaciens/pJL3 P19 infiltrated tissue from 3 7 DPI.
To better examine the potential for uncertainty we also examined growth rates using a stochastic population model [ 46].
Microarray technology offers the potential to examine the expression patterns of many genes simultaneously, thus gaining a better understanding of gene function, interaction, and regulation.
To examine the expression of candidate markers for micrometastasis.
DOI: http://dx.doi.org/10.7554/eLife.07103.016 To better examine gene expression and regulatory differences between human and chimpanzee iPSCs, we generated genome-wide RNA-sequencing and DNA methylation data (see 'Materials and methods') from all chimpanzee iPSC lines, as well as from 7 human iPSC lines also generated and validated in our laboratory.
In order to better understand the function of these genes, we firstly examined the expression of all the genes of subfamily IX of Arabidopsis in response to multiple environmental stimuli by means of microarray data available at Genevestigator site [ 68].
To understand better the functional role of PIK3R3 on cell proliferation, we examined the expression of various molecular markers in the PIK3R3 knockdown cells.
To better understand the role of PD-1 in antiviral immunity we examined the expression of PD-1 on Epstein-Barr virus (EBV) epitope-specific CD8+ T cells during acute infectious mononucleosis (AIM) and convalescence.
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