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To better examine the effect of FLCN expression on TFE3 post-translational modifications, FLCN and TFE3 were ectopically expressed in UOK257 cells using adenoviral vectors, and the cell lysates were separated by either 4 20% or 7.5% SDS PAGE (Fig. 3C).
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The effect of caffeine was also evaluated in Ca2+-free extracellular solution (2 mM EGTA) in order to better examine the effects of cholesterol depletion on Ca2+ release from the intracellular stores.
To better examine the effects of the primary blast wave only, exclusion criteria for bTBI were: (1) requirement for massive blood transfusion; (2) intracranial lesions causing mass effect; and (3) post-traumatic stress disorder (PTSD), because this has been linked with endocrine disturbance.
Moreover, in order to better examine the effects of spatial resolved PM10 in multiple-pollutant model, the other air pollutants also needed to be estimated spatially because between-pollutant relationships may not be characterized well just by the averaged value in one area.
However, some of these studies have been criticized for analyzing prevalent cases of type 2 diabetes and for differences of BMI between diabetic and non-diabetic subjects [ 24], recommending future case-control studies paired by BMI in order to better examine the independent effects.
Further, we assessed whether the metformin effect on the PCa incidence rate could be modified by statin use to better examine the heterogeneity of metformin effect.
In order to better examine the individual aspects of the effects of HIPEC, two groups were added in addition to group IV; these groups investigated the two noxa (cytostatics and hyperthermia) individually (group III: hyperthermic perfusion without MMC; group V: MMC as mono-therapy).
Furthermore, it is better to examine the effect of inter-lane interaction on crashes if the lane-based data is available.
Development of more complex models will be needed over time, and these models would be better suited to examine the effect of vaccination over a wide range of assumptions regarding vaccine coverage, efficacy, and duration of protection.
We examine the effect of contraception in preventing H.I.V. in Zimbabwean women.
All the laboratory studies I have seen examine how influenza survival and transmission vary with changing relative humidity conditions; none of them examine the effect of absolute humidity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com