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The optimized NLCs-based gel formulation showed superior texture, rheological profile and showed better cell uptake efficiency on hyperkeratinocytic cells (HaCaT cell lines) with higher ex vivo skin permeability efficiency vis-à-vis marketed formulation.
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The Efavirenz nanoparticles subjected for in-vitro cytotoxicity and cell uptake studies using monocytes / macrophages (THP-1) proved better uptake (Flocytometry and Confocal microscope) of nanoparticles than free drug.
Good cell uptake of nanocarriers can enhance the delivery efficiency and achieve better therapeutic effect.
'Better biochemistry, better cell biology, better genetics,' Nurse says.
Fig. 8 Cell uptake study.
Cell uptake is predominantly mediated by endocytosis.
Figure 1 Cell uptake and inhibition studies.
Figure 3 Cell uptake and washout study.
Fig. 3 Cell uptake of the nanoparticles.
Optimal uptake time will be selected for inhibition cell uptake studies.
Cell uptake of the nanoparticles was studied by confocal microscopy.
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