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First, selected candidate genes should have been validated by quantitative PCR (Q-PCR) and, if better, by western blots.
There has been debate in the epidemiologic literature as to which scale is better for assessing interactions (Blot & Day 1979; Saracci 1980; Rothman et al. 1980).
Since ELISA cannot reliably distinguish cleaved IL-1 β from pro IL-1 β, we used western blotting to better assess levels of cleaved IL-1 β.
BHMT, FAH, FBP1, DPYS and HPD need to be evaluated in larger clinical APAP overdose and acute liver injury studies using other protein detection methods besides western blots to better determine their sensitivity and specificity before they can be validated as translational and mechanistic biomarkers of liver injury.
We have performed additional IP/Western blot analyses to better characterize the interaction between LINKIN, RUVBL1, RUVBL2, and α-tubulin.
For both reporters, this leads to a better agreement with the Western blot measurements in the transition from exponential to stationary phase.
However, we feel that the original blots shown were better to bring the message across.
The heavier the caliper of blotting paper the better.
We agree with the reviewer that it would be better to show a single northern blot image with controls, duplications, and deletions all together.
For better comparison of Phos-tag western blots (αGFP) shown in different figures of this manuscript, all Phos-tag western blots were aligned here.
The protein extracts of 18 transformants repeatedly gave better results; this superiority was confirmed in Western blot analyses carried out using different sample dilutions.
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