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The delta beta value was calculated as difference in β-values between the two groups.
For each probe, a beta value was calculated, which approximately corresponded to the average methylation percentage in the sample analysed.
The raw methylation score for each probe represented as a methylation beta value was calculated using the GenomeStudio Methylation module software (2010.3) [ 34].
The Bead Chips were scanned using a Bead Station, and the methylation level (beta value) was calculated for each queried CpG locus using Methylation module of GenomeStudio software.
A beta value was calculated as the ratio of fluorescent signal intensity of the methylated probe to those of total (methylated and unmethylated) probes, and was considered as a DNA methylation level.
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Beta values were calculated as β = M'/(M'+U'), not using Illumina's offset.
Beta-values were calculated as M/(M+U).
Beta-values were calculated from methylated (M) and unmethylated (U) signal, as beta = M/(M + U).
A global X chromosome methylation value was calculated for each individual by summing the individual beta values at each of the 999 X chromosome CpG sites.
The Δ value was calculated by determining the mean value of beta-catenin expression by Real Time PCR.
A global methylation value was calculated for each individual in the study as the sum of the beta values at each CpG site, either across the X chromosome (global X chromosome methylation), or across all autosomes (global autosomal methylation).
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