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For the asthma-remission sub-group, the average within-twin beta difference was calculated, at these loci, by taking the average within-twin beta difference (beta value of asthma-affected twin (age 10) − beta value of unaffected co-twin (age 10)).
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Fold difference was calculated as 2−ΔΔCt.
The difference is calculated by SIScal − SISqi.
The differences are calculated.
Second, the absolute values of these differences were calculated.
Significant differences were calculated as described before.
Group differences were calculated.
The delta beta value was calculated as difference in β-values between the two groups.
In order to further understand the ecological processes behind beta diversity, a total beta diversity index was calculated.
Beta cell mass was calculated as pancreatic mass times the percent beta cell area for each mouse.
Electroencephalogram power within delta, theta, alpha, and beta frequency bands was calculated.
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