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Whether beta cells are obtained by direct reprogramming or from other sources, such as directed differentiation of pluripotent stem cells [ 41], it will be necessary to carefully characterize the phenotype of the cells before proceeding.
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Normal human pancreatic beta cells were obtained commercially HUM-CELL-0058 HUM-CELL-0058 HUM-CELL-0058ogy & Medicine Co., Ltd., Wuhan, China).
The 832 13 pancreatic beta islet cells were obtained from Dr. Tejal Desai (UCSF) at passage 51 and were grown in RPMI 1640 supplemented with 10% fetal bovine serum, 63.7 mg/L penicillin G, 100 mg/L streptomycin SO4, 1 mM sodium pyruvate, and 70 nM 2-mercaptoethanol (UCSF Cell Culture Facility) to approximately 70% confluency on 75 cm tissue culture plastic.
Real-time quantitative PCR analysis Highly purified primary mouse islet beta and alpha cells were obtained by fluorescence-activated cell sorting of transgenic mice expressing the fluorescent protein Venus selectively in the alpha cell under the preproglucagon promoter [ 34, 35].
THP-1 cells were obtained from ATCC.
HEK 293 cells were obtained from ATCC.
B65 cells were obtained from ECACC.
A673 cells were obtained directly from ATCC.
NTERA cells were obtained from LGC Standards, Germany.
HEK293 cells were obtained from ATCC.
It showed that when beta cells are destroyed, about 1percentt of alpha cells in the pancreas begin to look and act like beta cells.
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