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Pancreatic beta cell functionality is crucial for maintaining glucose homeostasis.
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Several key features of beta cell functionality were affected, including control of oxidative stress, glucose sensing, stimulus-coupling secretion and secretory granule biogenesis.
Pancreatic beta cell function was assessed by the HOMA for beta cell function (HOMA-B) [ 28].
OGTT-based indices of beta cell function were calculated.
Pancreatic beta cell function was assessed using the OGTT data.
ADCC-independent NK cell functionality was assessed in cytotoxicity assays against K562 cells.
Reduced expression of several transcription factors that play overlapping roles in establishing and maintaining beta cell functionality [ 5, 6, 8– 127 27, 44] was significant at P1 in the islets of Aldh1b1tm1lacZ null mutants whereas misregulation of other beta cell functional components was manifested mostly later.
Their expression levels are also important for beta cell functionality [ 5– 12].
We generated an Aldh1b1-knockout mouse line (Aldh1b1tm1lacZ) and used this to study pancreatic development, beta cell functionality and glucose homeostasis in the absence of Aldh1b1 function.
CD4+ T cells functionality was evaluated by anti-OVA IgG titration (Figure 6B).
So prevention may come before restoration of beta cell function, that's cool, steps matter".
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