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Considering the effect of BET proteins in a plethora of transcriptional physiological and pathophysiological processes, it is no surprise that at higher concentrations of both CBP inhibitors, a BET effect is the dominant fingerprint of the compound [ 45, 46; P icaud et al., U npublished D ata].
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By contrast to STAT1 and STAT4, the T-bet-inducing effect of IFN-α mainly concerned the naive subset (Fig. 2B).
Interestingly, the T-bet-inducing effect of IFN-γ observed here appeared to last longer than that of IFN-α (Fig. 3A) and affected naive and memory subsets to similar extents (Fig. 3B).
For the investigation of bet size effects, we therefore collapsed the 10- and 50-point bets together in comparison against the high bets (90 points).
Altogether, our data strongly suggest that, in the context of ESCs, BET inhibition effects are mostly due to BRD4 displacement and that, of all BET members, BRD4 acts as the most important regulator of ESC identity.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com