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For each gene, the best unambiguous hit was retained.
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In Olympic events the best is unambiguous.
The Pap3 search revealed an unambiguous best hit in Manduca sexta, with Pap2 and a number of hemolymph proteinases as alternatives within the same species, but with considerably less similarity.
In addition, the following uniqueness criterion was imposed to make sure that the two best alignments were unambiguous: for a query with three or more alignments, the alignments ranking third or worse were compared with the second best alignment (constituting the "short" partner of the reconstructed transcript).
In some circumstances, only the unambiguous best alignment for each read is required, rather than the full catalog of all alignments.
It is modeled after the short read-mapping program RMAP, and reports either all alignments or the unambiguous best alignment for each read with any number of mismatches or differences.
If multiple genes produced identical bit scores with a given contig/singleton, ties were broken as follows: i) if exactly one of tied genes gave unambiguous best hit with some other contig(s) or singleton(s), this gene was selected, ii) in the remaining cases ties were broken randomly.
Your organization performs best when strategy is unambiguous.
Nature's best thermometer and most unambiguous indicator of climate change is ice.
Younger than the typical Higgins (but more the age Shaw imagined), Mr. Hadden-Paton, best known for playing unambiguous good guys on "Downton Abbey" and "The Crown," makes sense of the character's on-off switch of vulnerability and hauteur.
The reduce function scans the list of alignments for each read and records the best alignment if an unambiguous best alignment exists.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com