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Best quality crystals, as judged by eye, were observed at 0.5 and 1 mol % DOPC.
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While the crystal-picking method by Fujita and co-workers can be performed, where a crystal is selected by visual inspection prior to the soaking experiment (Inokuma et al., 2014 ▶), it is highly recommended that multiple soaking experiments are set up in parallel in order to increase the probability of finding the best quality crystal as judged by reciprocal-lattice evaluation.
The best diffraction quality crystals were obtained with the reservoir solution containing 180 mM ammonium sulfate, 100 mM sodium acetate pH 4.5, 1 mM calcium chloride, 20% PEG 4000 and 3% D-glucose.
An optimization procedure yielded the best diffraction quality crystals, which were obtained by mixing 1 μl of the protein solution with an equal volume of a reservoir, containing 0.1 M 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid (HEPES) (pH 7.5), 0.2 M magnesium chloride and 24- 2-hydroxyethyl -1-piperazineethanesulfonic
Further optimization by equilibrating 1 µl CrSPI-1-D1 protein solution of 15 mg/ml and 1 µl reservoir solution (0.4 M mono ammonium dihydrogen sulphate, 0.1 M Tris-HCl pH 8.5) using hanging drop vapor diffusion technique at 20°C led to best diffraction-quality crystals.
After further optimization, crystals of the best quality were obtained after 1 week, with the maximum dimensions reaching 0.35 × 0.05 × 0.05 mm.
Various parameters of the growth sequence were studied and the microwave power density was found to have the major influence on the crystal morphology, with the best quality diamond formed under conditions of relatively high pressure and plasma density.
"The best quality," Mr. Abdul said.
What is your best quality?
[Six.] What's Jeremy Corbyn's best quality?
Her youthful confidence is her best quality.
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