In cell cultures in LB medium, for the two concentrations of IPTG tested (0.5 mM and 1 mM), the best expression levels were obtained after 4 hours of induction, with similar band intensities for both concentrations (Figure 4).
When the fusion partner BRIL is inserted at ICL3, Glutamate family receptors collectively produced the best expression levels, although Rhodopsin family receptors constitute the majority of the receptors whose surface expression levels exceed 80% (Fig. 4).
Treg are best characterized by high expression levels of the IL-2 receptor α-chain (CD25) [ 9, 10].
To select the best reference gene, the expression levels of the twelve genes were measured in 32 glioma samples using real-time PCR.
Also, in order to assess applicability of the targets for human molecular imaging studies, we identified articles using a stringent or high cut-off value and preferentially membranous staining localization, as these studies provide the best evidence for high expression levels of the target.
To the best of our knowledge gene expression levels of MMP-28 have not been investigated in periodontal diseases.
Genes with lowest M value are the most stable and geNorm estimates the normalization factor (NFn) using geometric mean of expression levels of n best reference genes.
Given our previous result showing that genes with low expression tended to show poor biological reproducibility, we next compared the expression levels of our best probes that passed compared with those that failed.
The Visual Basic Application geNorm version 3.4 [ 5, 14] was used to evaluate the expression stability of each gene, determine the ideal number of genes required for normalization, and calculate individual normalization factors based on the expression levels of the best reference genes.
To assess radiation-induced response of protein markers in human lymphocytes irradiated ex vivo, we measured expression levels of our three best candidates, FDXR, DDB2 and ACTN1 protein, 3 days after initial exposure (Fig. 4B).
