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The best evolutionary models were chosen by applying a hierarchical likelihood-ratio test using MrModelTest 2.2 [ 34] for the rDNA sequences, and applying a mixed model to translated mtDNA sequences.

The best evolutionary model was also determined with MEGA5.

In all cases the best evolutionary model was estimated to be JTT + G (data not show).

Identification of the best evolutionary model is performed via the AIC approach (see above).

For each case the best evolutionary model was estimated using MEGA5 [ 64].

The best evolutionary model was estimated for each alignment using MEGA5 software [ 67].

The best evolutionary model was estimated with GARLI software (i.e., general time reversible + Γ + I model).

The best evolutionary model was predicted by jModelTest [ 51], and the Bayesian inference (BI) was evaluated with BEAST 2.1.3 [ 52].

Selection of the best evolutionary model is based on the same AICc approach described above for model-selection of lineage accumulation.

For both the 12S and 16S datasets, the best evolutionary model was GTR+I+G, whereas for the cytB fragment, it was TIM2+I+G.

Finally, for the Rhod fragment, the best evolutionary model was TPM3uf+I+G (Table  1).> The Bayesian analysis resulted in a monophyletic and highly supported Adenomera clade with Lithodytes lineatus as a sister species.