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The best evolutionary model of nucleotide substitution was determined using jModelTest 0.1.1 [ 102].
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The best evolutionary model for each of the sequenced genes were the six parameter general time reversible (GTR) model of nucleotide substitution (nst = 6, rates = invgamma).
The best evolutionary model for each of the selected partitions was simultaneously estimated in the same software.
Version 3.06 of Modeltest [25] was used in conjunction with PAUP* 4.0 [26] to estimate the best evolutionary model for the construction of each maximum likelihood (ML) tree using the Akaike Information Criterion [27].
The tree obtained by NJ was refined using the tool PROTTEST [ 46], to determine the best evolutionary model for the set of query proteins [ 47].
Using the hLRT method of the program ModelTest 3.06 [ 32], the ML model of GTR+G was selected as the best evolutionary model for the combined data set.
To test for the best evolutionary model for the analyses, the log-likelihood values of 56 models using Modeltest 3.7 were compared.
The best evolutionary model for sequences derived from each patient included a class allowing for positively selected codons (ω>1) in 18 of the 22 analyzed patients (Table 3).
The best evolutionary model for the ML analysis was selected by the ProtTest [ 52].
The best partition scheme selected was as follows: the 1st and 2nd codons of tef1α were defined as one partition, with K80 + I + G as its best evolutionary model; the 3rd codons of tef1α, and the 1st, 2nd and 3rd codons of rpb1 each represented a separate partition, with HKY + G, TrNef + I + G, SYM + I and HKY + I + G as their best evolutionary models, respectively.
Selection of the best evolutionary model is based on the same AICc approach described above for model-selection of lineage accumulation.
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