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Identification of the best evolutionary model is performed via the AIC approach (see above).

Selection of the best evolutionary model is based on the same AICc approach described above for model-selection of lineage accumulation.

The best evolutionary model was also determined with MEGA5.

In all cases the best evolutionary model was estimated to be JTT + G (data not show).

For each case the best evolutionary model was estimated using MEGA5 [ 64].

The best evolutionary model was estimated for each alignment using MEGA5 software [ 67].

The best evolutionary model was estimated with GARLI software (i.e., general time reversible + Γ + I model).

The best evolutionary model was predicted by jModelTest [ 51], and the Bayesian inference (BI) was evaluated with BEAST 2.1.3 [ 52].

For both the 12S and 16S datasets, the best evolutionary model was GTR+I+G, whereas for the cytB fragment, it was TIM2+I+G.

Finally, for the Rhod fragment, the best evolutionary model was TPM3uf+I+G (Table  1).> The Bayesian analysis resulted in a monophyletic and highly supported Adenomera clade with Lithodytes lineatus as a sister species.

The best evolutionary models were chosen by applying a hierarchical likelihood-ratio test using MrModelTest 2.2 [ 34] for the rDNA sequences, and applying a mixed model to translated mtDNA sequences.