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Following preliminary dose-response experiments (not shown), the best effective concentration of 100 µg/ml of each mAb was selected.
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The non-linear regression function for sigmoidal dose-response (variable slope) of the GraphPad Prism software was used to calculate the (best-fit) effective concentration (EC50) values.
We reasoned that such a low but effective concentration could best make the point regarding concern about prevalent environmental contamination levels.
In our data the EC50 (median effective concentration) from the best fit regression relationship is approximately 10 mg/kg, which is much lower than the 33 mg/kg estimated from effects on mink used as a surrogate species in the risk assessment study by Schwacke et al. (2002) on bottlenose dolphins.
Among these substances, compound 6r showed the best inhibitory effect against Xoo, and its half-maximal effective concentration (EC50) value is 4.78 μg/mL, which is superior to that of commercial agent bismerthiazol (87.55 μg/mL).
At the highest concentration used, NRG4 promoted branching as effectively as the maximally effective concentration of NGF and acted over a similar developmental time course as NGF.
Among the title compounds, 7j exhibited the best curative activity against TMV, with a half-maximal effective concentration (EC50) value of 213.5 μg/mL, which was better than that of ningnanmycin (270.9 μg/mL).
Furthermore, the in vivo 50% effective concentration calculated from these data best matched GSK501015's in vitro potency when serum protein binding was accounted for.
A concentration-response analysis of glycine-mediated currents was best fitted by the Hill equation and yielded a half maximal effective concentration (EC50) of 69 ± 12 μM, a Hill coefficient of 1.4 ± 0.3, and an average maximal current of 270 ± 73 pA.
Effective concentration (EC50) values were determined by dose-response analysis and best fit curves generated by nonlinear regression analysis also in GraphPad Prism.
The effective concentration killing 50% of CLL cells (EC50) derived from the equations that best fit the linear range of the dose-response curve.
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