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Exact(4)
In order to evaluate the real-time assay and to determine the best dilution value between the first and second reaction, a 1∶2 serial dilution (2.0×10−4 to 7.81×10−7) of PCR product from the first reaction positive control (pox culture lysate) was prepared and run using the real-time conditions described above.
Preliminary experiments were made with three dilutions of each cDNA samples to find the best dilution for the relative quantification.
The 1 150 dilution was the best dilution compared to 1 50, 1 100, and 1 200.
A ratio of less than two suggests either saturation or loss of detectability, and thus the best dilution is the one that yields the highest signal intensity in the linear regime of the dilution curve.
Similar(55)
This work addresses the best effluent dilution (WC, %) as well as sodium bicarbonate concentration (SBC) on the alga growth and biochemical composition.
It was found that; methanol was the best solvent for dilution as it gave the highest sensitivity with good shaped spectrum.
It is perhaps the underground's indifference to profit-based motives that is its best protection against dilution by popularity.
The particle concentration on the grid is best adjusted by dilution of the sample solution.
Following selection of the best clones serial dilutions were prepared to show correlation between cell number and photon output.
The concentration of BACE substrate used was that which best differentiated serial CSF dilutions over different time points.
No significant differences were observed in relation with the sample dilutions tested, although the best ICT values were obtained with 1/10 dilution (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com