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Similarly, we determined the best clock model for estimating the evolution rate of the gag gene.
We first evaluated the marginal likelihood of the three clock models employed in this study to determine the best clock model for estimating the evolution rate of the pol gene.
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The resulting BSPs were compared by Bayes Factors [22] and the best clock model was selected for the estimation of tMRCA.
Once again, the best clock model was selected by Bayes Factor.
The trees obtained with the best clock model were analyzed with BaTS in order to calculate the Parsimony Score (PS), the Association Index AII) (for overall association) and the Monophyletic Clade (MC) (for each state) statistics.
The best clock model was assessed in every case using BF comparisons as above.
Once the best clock model was identified, lineage ages were reestimated using the best clock and partition scheme P1, but selecting the Birth-death speciation process as a tree prior.
The uncorrelated lognormal clock model was determined to be a significantly better fit than the strict clock model for each of the datasets (logBayes factors ≫ 2).
We used all individuals for which full phase information was available and assumed a strict molecular clock model for all loci and made used of the best-fit model for each partition, as determined with DT_M ODSEL[ 43].
A significant difference (P<0.001) between the log likelihood values of clock-like versus non-clock-like behavior justified the use of the relaxed molecular clock model for the clade of East Asian Cyprinidae.
Clock rates in substitutions/site/Myr x 10−4 estimated by BEAST under each clock model for selected clades.
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