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In the axtTight track, mouse and human genomes were aligned using BLASTZ [ 32] and post-processed to obtained the best alignments for each region.
As an optimization, the reducers in the main alignment algorithm report the top two best alignments for each read.
Reads were aligned to the yeast genome (sacCer3 assembly) with BOWTIE [ 49], allowing only unique and best alignments for each read.
For this purpose the mouse-human genome alignments were taken directly from the UCSC genome annotation project [ 31], where the two genomes were aligned using the BLASTZ software [ 32] and post-processed to obtain the best alignments for each region (see Methods for details).
Exonerate was set to report the five best alignments for each contig and to output a table showing, for each alignment, the names of the contig and the reference, the beginning and end of the aligned region in each, and the score and percent identity.
Similar(55)
The reads are aligned to both genomes, and the best alignment for each read is retained.
After alignment to these converted genomes, B-SOLANA determines the best alignment for each bisulfite sequence in the following way: bisulfite sequences that are aligned to different genomic positions in Conversions I and II are assigned to the position with the lowest number of mismatches.
Only the best alignment for each sequence was kept.
The best alignment for each transcript was retained as annotation.
The first analysis only selected the single best alignment for each long read.
Then on each single patch, a complete search is conducted to guarantee the best alignment for each mimotope sequence.
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