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Also the best active fraction (FV) was functionally and structurally characterized.
The most cytotoxic fraction, that is, best active fraction (BAF), was further evaluated for in vivo anticancer potential after its quality control analysis, using HPTLC.
The cytotoxicity assays of mother extract and its hexane, DCM, n-butanol, acetone, methanol, and water fractions were carried out to find out the best active fraction (BAF).
Hydroalcoholic extract of Picrorhiza kurroa and its fractions were subjected to in vitro screening for cytotoxicity; further best active fraction (BAF) obtained was tested against Ehrlich ascites carcinoma (EAC) model in Balb/c mice after its quality control analysis.
The DCM fraction was found as best active fraction in in vitro testing with lowest IC50 value (36 51 μg mL−1 at 72 h) among the four tested cell lines.
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The best separation of the active fraction was achieved using TCA precipitation followed by fractionation using Superose™ 12 gel filtration.
The best activity was obtained for the crude water extract (53.69 ± 2.36) and the least active fraction which gave significant inhibition was n-butanol (24.31 ± 1.09).
The percentage inhibition of AM ranged from 28.03 ± 0.45 to 82.46 ± 10.09, and the best activity was observed for the crude water extract 82.46 ± 10.09 and the least active fraction which gave significant inhibition was the n-butanol fraction.
Chloroform fraction was most active fraction as it showed considerable activity against all bacterial strains.
The active fraction was eluted by 30% aqueous methanol.
The active fraction was used as the purified enzyme solution.
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