Later, many researches suggested that the miRNA repertoire of plant species comprises a set of non-conserved miRNAs besides the conserved ones [ 27- 29].
Surprisingly, besides the conserved residues in the two helices, such as Leu229 and Leu249, Ala232 and Ala252, and Ser236 and Ser256, the chemical shifts of the linker residues (Gln238, Glu239 and Ile240) are also strongly perturbed.
The model of RelE2Spn also sheds light on some possible structural peculiarities of the toxin: unlike its E. coli and P. horikoshii homologues, RelE2Spn would include in its catalytic site (besides the conserved R residues), residues H43, Y31 and Y57, and two acidic residues (E38 and D39) (Figure 7 and Figure S4).
Therefore, besides the conserved catalytic domain, the additional domains also play important roles in functions of kinases.
Division III consists of class VII CHS that have the simplest domain structure and carry no other domains besides the conserved CHS catalytic domain (pfam03142) present in all CHS (Choquer et al. 2004; Riquelme and Bartnicki-García 2008).
Six additional syntenic segment associations (that is, HSA 3/6, 5/19, 8/9, 10/15, 16/20 and 18/22/12) were present in the NPH genome besides those conserved syntenic segment associations ancestral for eutherian mammals.
This, together with the finding that hybridizations with telomeric (TTAGGG) sequences as probes revealed telomere-only distribution patterns on the chromosomes of M. californicus and M. waterhousii, indicates that besides the conserved telomeric sequences, the chromosome termini of MCA are comprised of large amount of repetitive DNA, which might be unique to this species [ 25].
The networks of agricultural entrepreneurs have contributed to the sustainable development of the region studied, since besides economic benefits, they have contributed to conserving the landscape, job creation and preserving traditions.
