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Several studies have demonstrated significantly lower ADC values in prostate carcinoma compared with benign prostatic tissue[ 50 53], suggesting clinical utility in prostate cancer detection.
This hypothesis was supported by our results from TMA studies of primary prostate cancer and benign prostatic tissue from 448 patients.
Clinical reports indicate that Akt is significantly over-expressed in prostate tumors compared to benign prostatic tissue, and its level is directly correlated with tumor progression and prostate-specific antigen (PSA) serum levels, as well as a higher Gleason score [ 23, 26].
As for the other histological findings, the most common was chronic prostatitis (51%), followed by malignant neoplasia (27%), benign prostatic tissue (11%), atrophy (5%), chronic prostatitis with secondary acute prostatitis (4%), and prostatic adenomatous hyperplasia (2%).
In prostate cancer, abnormal diploid cancers may represent an early stage in ploidy progression and DNA ploidy abnormalities occur in benign prostatic tissue adjacent to many prostate cancers [27], [28].
For immunohistochemistry paraffin sections of tissue microarrays comprising prostate cancer and corresponding benign prostatic tissue samples were treated with xylene and ethanol.
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By comparing PUFA content from malignant and benign prostatic tissues from the same prostate specimens, a Swedish research group found that n-6 PUFA and n-6 PUFA precursors were significantly higher in malignant tissues.
Metastatic prostate cancers (n = 20), hormone refractory prostate cancers (n = 19) and benign prostatic tissues (n = 38) were also analyzed.
The staining intensity of MIC-1 protein in prostate adenocarcinoma samples was scored and compared with normal and adjacent benign prostatic tissues, and the value was considered enhanced if the staining intensity was higher by one or more points.
The use of longer b values (b ≥1000 mm²/s) facilitates the detection of cancer foci compared to the benign prostatic tissues [63, 64].
Furthermore, the staining intensity of the secreted MIC-1 protein detected in the stromal compartment was substantially enhanced in PC tissue specimens as compared with normal and adjacent benign prostatic tissues.
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