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Metastasis distinguishes malignant tumour cells from benign and normal cells and is the main cause of short survival and high mortality for cancer patients (Mareel, 1984).
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Whereas COX-2 is weakly expressed in most benign tumours and normal cells, it is overexpressed in malignant pheochromocytomas (Salmenkivi et al, 2001).
Higher expression levels of the ERK1/2-specific cytoplasmic dual specificity phosphatase 6 (DUSP6) in comparison to benign and normal thyroid cells has been previously associated with PTC, especially with advanced thyroid carcinomas [ 30, 31].
The MCF10A (ERα-negative) and 184A1 (ERα-negative) human cell lines are immortalized benign and normal breast epithelial cell lines, respectively.
Cell differentiation and drug-induced differentiation of tumor cells into benign or normal cells, are important targets for anticancer chemotherapy [ 5].
RNA was isolated from frozen tissue for 64 tumour samples, all benign and normal samples and 1 × 105 cells from each breast cell line using Tri-reagent (Sigma).
Our data showed that miR-133a was predominantly expressed in luminal epithelial cells in both ductal and lobular structures in benign and normal breast specimens.
Prostate cancer cells exhibited a pronounced global decrease in methylation compared with benign and normal tissue.
The cancer cases were consecutive; the benign and normal cases were randomly selected.
Recent computational models suggest that hypothetical benign cell boosters, which increase the fitness of either benign neoplastic clones or normal cells, may help to drive the more dysplastic clones extinct and thereby delay cancer progression.
The intensity of testosterone-BSA-FITC staining was different depending on the nature of cells examined: for normal cells and benign prostate hyperplasia the staining was very low, while cells from prostate cancer were intensively stained.
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