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Multiple regression analyses in a phylogenetic framework [17], [18] as described below were performed on PC1 and PC2 (which together explained 80% of the variation in hook shape).
Haematoxylin-eosin staining and immunohistochemistry, described below, were performed on all surgical specimens.
All further analyses below were performed on the 2,632 tumor-expressed lncRNAs (Additional file 2).
Both laboratory analyses and the clinical examinations described below were performed on two occasions, directly before and after the rosuvastatin treatment period.
While the analyses below were performed on both ν and π, results show that ν or π can be used almost interchangeably without affecting the conclusions.
Then, behavioral tests indicating locomotor and pain-related behavior (see below) were performed on days 1, 7, 14, and 21 after treatment.
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The analysis described below is performed on the isochore sequences with masked repeats.
The statistical analysis described below was performed on the ERP components isolated in the first block of experiments for participants with valid data.
ESI-MS (see below) was performed on hTTP peptides that were from solution digests or extracted from polyacrylamide gels with and without MOAC enrichment.
Then the new protocol for fluorescence detection, described in this manuscript (see below), was performed on the stripped membranes.
PCR product detection, using the Limk primers listed below, was performed on the ABI PRISM 7700 system (Applied Biosystems), under standard conditions and normalised with a GFP control.
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