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All assays below were performed in serum free media whenever appropriate to ensure that no exogenous OPN was added.

Given these data we decided to perform our study in the severe contusion injury model; studies reported below were performed in severe contusion as defined by Basso et al. [3].

Most geometric morphometric analyses (except between-group PCA, see below) were performed in MorphoJ [ 44].

All statistical analyses described below were performed in SAS, and Bonferroni correction was applied for each analysis.

The electrophysiological tests mentioned below were performed in all patients: full-field electroretinogram (ERG), pattern electroretinogram (PERG) and pattern visual evoked potentials (PVEP).

All experiments described below were performed in accordance with local and national guidelines governing animal experiments (86/609/CEE and its modifications).

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After the preparation of fed and unfed nubbins, a set of initial measurements, described below, was performed in all tanks (called day 0, see Supplementary Information).

Survival analysis (see below) was performed in a homogeneous subgroup.

The experiment described below was performed in order to test this prediction directly.

We recommend that more qualitative checks, described below, be performed in order to assess the approximation quality.

Graphs depicting the evolution with time of apparent bubble cross-sectional area, from pre-compression, through compression and gas shifts, until the total dissolution of the bubble were first inspected visually for deflection points, trend reversals, obvious changes in slope, etc. Bubble analysis (see below) was performed in a random order, unblinded to the observer.

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