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Additionally, 2.5 pg (0.25 pg/μl) of in vitro synthesized and polyadenylated cyc3GFP mRNA (see below) was added to each sample.
The electrophoresis mobility marker solution (10 μL; preparation is described below) was added to each sample in the 96-well plate and dried in a CVE.
Subsequently, 50 μL of mobile phase B (described below) was added to reconstitute the residue, and 5 μL of the reconstituted sample was injected into the HPLC system.
For each sample 10 μl of 2× Orange G loading buffer (composition shown below) was added to 5 μl of the purified PCR product and made to 20 μl with water.
The statement below was added to address this issue: "Using a colorimetric assay to quantify larval glucose levels, paroxetine incubation from 3-5dpf demonotrateffecteffect on larval glycaemia – albeit transient increases on days one or two of treatment cannot be excluded".
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To prove this PO, guard grd3 below is added to the AddAuthorisation event to ensure that each role is linked to a data item that actually belongs to a data source.
These habitat pieces (and also for the sex ratio experiment, below) were added to make a more familiar surrounding for the animals, however, it was so little substrate that it did not infer with isopod movement or allowed for hiding etc. which could have affected the results.
LBGs, and presence of parabasal cells and leucocytes (see below) were added to the final AV score [ 20].
The amiRNA constructs from pRS300 derivatives (see below) were added to pCAMBIA 1300 35SP-erGFP7INT-NOSS using BamHI/PstI sites.
Respective phospho antibodies as listed below were added to the cell pellet and allowed to incubate for one hour at ambient temperature in the dark.
Different competitors (described below) were added to the mixture to demonstrate the specificity of binding of MAF1 at the Pol III promoter element.
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