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One of the late colonizer strains was an early colonizer strain that had remained in the infant for four months (see below), this strain was considered an early colonizer and the enrichment criteria were modified to minimize bias.
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In NCM4781 we found a yehD mutation, despite the fact that sequence coverage in this strain was low (see below), which led to more than double the number of false positives seen in other strains and a complete lack of sequence coverage in some regions of the genome.
Strain BY4741 consumed all glucose within 48 h at n-butanol concentrations below 1.1%, the CEN.PK113-7D CEN.PK113-7Dble to constrainll glucose up to a butanol concentration of 1.3%.
For initial angles below the neutral angle, creep strain was shown to facilitate a shift in orientation towards the neutral angle.
For the results described below these strains are considered together.
Burr et al. measured the strain by surgically implanted strain gages and found that the strain was below 2000 microstrains even under conditions of strenuous activity [ 23].
Whereas the amount of tRNA was similar in both strains, the Q content of tRNAAspGUC from the qtrt1Δ strain was below the detection limit of our experimental setup (Supplementary Figure S6B), validating both the role of eTGT in Q salvage in S. pombe and our experimental approach.
The crude amylase of the AS22 strain was stable at temperatures below 40°C.
The results of SEM, FEM, and micro-hardness tests indicated that under the pure rolling contact condition, the maximum plastic strain was approximately 200 400 μm below the contact surface.
A USA300 ΔhlgABC isogenic deletion strain was constructed as described below.
The recA gene of the CIChE strain was then deleted (see below).
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