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Furthermore, amplification efficiency monotonically decreases as DNA concentration increases, in contrast with the assumption that amplification efficiency is constant below the quantification threshold.
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The first change – the removal of the quantification threshold – could have a major effect on federal agency practice.
CEQ removed the quantification threshold and now recommends that agencies quantify direct and indirect GHG emissions whenever the tools, methodologies, and data are available to do so.
Extracts whose amplification was below the threshold level of quantification of 100 IU/mL were confirmed to contain HEV RNA through amplification in a second PCR by using inner primers JVHEVF and JVHEVR (17, 18 ).
In contrast, relative amounts of all of the LHR variants in GC taken from follicles <6 mm, was below the threshold for detection or accurate quantification.
This loading is below the percolation threshold for electrical conductivity.
However, both Pakistan and India have explored options below the nuclear threshold.
Above the highest threshold, and below the lowest threshold, no plasticity occurs.
(d) T = 0.97, just below the percolation threshold.
There are 476 schools below the 60% threshold.
The fainter rings are still far below the visibility threshold.
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