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At concentrations significantly below the minimum gelation concentration, spherical aggregates are observed.
PBLG nanofibers form at concentrations well below the minimum gelation concentration.
Static light scattering and TEM images reveal the formation of nanofibers upon the addition of alkaline phosphatase, even at concentrations (e.g., 200 μM) below the minimum gelation concentrations (mgc; e.g., DDD- 1: 1.0 mM).
The results of static light scattering and TEM images show the formation of nanostructures upon the addition of alkaline phosphatase, even at concentrations below the minimum gelation concentration (mgc).
For samples below the minimum gelation concentration (Cgel), the specimens were prepared by placing one drop of the clear solution onto the carbon-coated copper grid, removing the solvent with a tissue and air-drying the sample for 1 min with an airflow.
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The most efficient gelator is Fmoc-4-fluoro-phenylalanine, which can gel PBS buffer solution at the minimum gelation concentration of 0.15 wt%.
The micelles at the concentrated solution formed physical gel, and the minimum gelation concentration of the triblock micelles was lower than that of the corresponding diblock micelles.
The lower the molecular weight of the PBLG, the higher the minimum gelation concentration.
The minimum gelation temperature was observed for batch VG9, that is, 24.36 ± 0.41.
As a characteristic and easily accessible parameter for this type of gel, the minimum gelation concentration (Cgel) is determined by varying the concentration and performing the vial inversion test (Fig. 1).
The minimum gelator concentrations (MGCs) for the gelation of ionic liquids depend on the chemical structures of the gelators.
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