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In general, the PCR temperature profile was 30 cycles of 94°C for 60 s, annealing temperature set approximately 5°C below the lower of the two primer melting temperatures for 90 s, 72°C for 150 s, and a final 15 min elongation period at 72°C.
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As suggested by the relative frequencies in Figure 3 and further discussed below, the lower end of the QALE scale is the primary source of this disagreement.
More worryingly, while the next five highest prices greeted works that raised no questions whatsoever, these too sold at, or below, the lower end of the estimate.
In each of the other groups a number of animals had levels below the lower limit of detection of the assay.
Method blanks were below the lower limit of determination of 5 ng/g for Hg analysis of these solid samples.
Method blanks were below the lower limit of determination of 0.01 ng/g for methyl-Hg analysis of these solid samples.
Cytokine measurements below the lower limit of detection of the assays were allocated an arbitrary value of 1 ng/ml to permit intergroup statistical analysis.
Insulin measurements below the lower limit of detection of 2 mIU/L (n = 1) were left out of the analysis.
Low copy number oral HPV infections below the lower limit of sensitivity of the line blot would not be selected.
Field and method blanks contained DOC below the lower limit of determination of 0.3 mg/L.
Insulin measurements below the lower limit of detection of 2 mIU/l (n = 74) were set at 2 mIU/l.
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