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Optical images revealed a spatial resolution well below the diffraction limit.
Surface plasmon resonance (SPR) by the electric field along a metallic surface can confine optical excitation to far below the diffraction limit [12, 13].
By moving the focus of the laser through the sample in three dimensions and determining the position of the fluorescence maximum for each molecule, the group could pinpoint its location with an accuracy well below the diffraction limit.
By reducing the cross-sectional area of the focal spot by about a factor of 18 below the diffraction limit (260 nanometers), STED allowed us to map and describe the vesicle mobility within the highly confined space of synaptic boutons.
These include negative, near-zero, and indefinite permittivity or permeability indices; backward waves and backward phase matching in nonlinear optics; and potential applications of metamaterials for perfect absorption, cloaking, and imaging below the diffraction limit using super- and hyperlenses.
The increased resolution afforded by SIM images allowed to resolve the mini-rings below the diffraction limit and to investigate the spatial relationship between circular and linear FtsZ assemblies.
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Accurate optics-based dimensional measurements of features sized well-below the diffraction limit require a thorough understanding of the illumination within the optical column and of the three-dimensional scattered fields that contain the information required for quantitative metrology.
Despite the revolutionary significance, the conventional far-field light microscopy is restricted by the diffraction limit based on the scale diffraction theory; that is, objects within a diffraction-limited spot, ∼250 nm, cannot be resolved.
One way to get around the diffraction limit is to stick a tiny light source very close to the sample.
Usually, optical microscopes cannot make out details smaller than half the wavelength of light, or about one-100,000th of an inch, a constraint known as the diffraction limit.
In 1995, Eric Betzig, of NSOM Enterprises, proposed a way to get around the diffraction limit.
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