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The number of probesets was reduced to 14'509 by removing probesets expressed below the defined background threshold.
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The data were then filtered to remove genes deemed to be expressed at a level below reliable quantification by determining those with a raw signal value below a defined background cut-off value of 100 in all samples.
However, SOX11 negative cell lines as JVM2 (MCL), MOLT-4 (Acute T-lymphoblastic leukemia), RL and DOHH-2 (both FL) generated only background signals, below the defined cut-off, in accordance with mRNA expression data.
MC defined the background of the study.
Below, we define the score for each block.
Spots with a volume below 50,000 were defined to be background.
Arrows indicate single-step photobleaching events that define the background of our smFRET assay.
Return the data and preferences defining a background plot.
The entire human proteome was defined as the background.
For the other particles biodegradability was below the normal background fluctuation of 5%.
The results showed that the appropriate threshold is slightly below the local background intensity.
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