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All genes had high expression stability (M-values <1), below the default limit of M = 1.5, except for GAPDH.
According to the published articles [ 4, 30], stable genes' M values were below the default limit of 1.5 in the geNorm program.
All six genes showed high expression stability and had M values lower than 0.8, below the default limit of 1.5 suggested by the geNorm program.
Genes with an M value below the default limit of M = 1.5 were considered as having acceptable expression stability (or suitability as normalizing gene) and genes with the lowest M values were taken as having the most stable expression [ 43].
All the tested samples reached a high expression stability with relatively low M values of less than 1, which are far below the default limit of M ≤ 1.5 [ 14].
The analysis by geNorm suggested that all studied genes reached a high expression stability with low M values (Table 4 and 5), below the default limit of M = 1.5[ 1.5[
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Twitter is taking the default limit from 350 and cutting it to 175.
Generally, reference genes with geNorm expression stability measures below 1.0 have been regarded as suitable for normalisation in some studies [ 28, 39, 44] and are also below the geNorm default limit of M = 1.5 [ 6].
All hits below the default RPSBLAST e-value of 1e-03 wereportedted [ 37].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com