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Although NOXA expression was inducible in all cell lines in response to 5-FU (∼4-fold) at 48 h, its expression in KYSE70 and KYSE450 was still well below the basal expression levels in OE21 cells suggesting there may be a deficiency in BH3-only signalling.
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Interestingly, without the palmitate treatment, Yap1 knockdown did not influence the basal expression level of CTGF (Fig. 4E and 4F).
GA treatment managed to prevent the diet-induced RAGE expression dysregulation in the aorta, AM and SAT without jeopardising the basal expression in all the studied tissues.
In our experimental conditions, CDKL5 protein levels in SKNBE cells were below detection limits (data not shown), confirming the low basal expression of CDKL5 in SKNBE compared to SH-SY5Y cells.
However, this may be related to the slightly lower basal expression of c-Jun that was observed in the myelinated cultures from the Hsp70 KO mice (see below and Figures 6 and 7).
The EsmtMnSOD with low basal expression level responded to invading microbes intensely, while the EscytMnSOD with high basal expression level exhibited mild responses against stimulating microbes.
At a concentration of 5 µM, GSI inhibited ligand-dependent activation, and suppressed expression below the basal level observed in co-culture with the parental 3T3 Flp-in cells, as might be expected based on the ability of GSIs to block the activity of all Notch family members expressed on the tumor cells (Figure 4).
As shown in Fig. 1F (lane 3) and Fig. 1H (lane 3) rapamycin attenuated KCC4 mRNA expression below the basal level, following treatment with either VEGF or PlGF.
We processed the tissue such that low levels of basal expression in WT animals were below the detection threshold of this technique (as demonstrated by the very low staining intensity in WT tissue).
The expression host also bore the pLysS plasmid to repress basal expression.
However, as negative regulation for some genes was ignored because the down-regulation of genes cannot push gene expression levels below the basal level, some organizations represented biologically infeasible system states, for example the simultaneous uptake of all carbon sources.
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