Sentence examples for below the background hybridization from inspiring English sources

Previous microarray studies typically filtered out all genes with expression below the background hybridization threshold, effectively removing loci with low or no expression.

The mismatch oligonucleotide provides an estimate of the background hybridization signal.

Baumgart et al. also showed that acetylation of tissue sections could reduce the background hybridization (1).

The data were screened to remove weak signals below the level of background hybridization in both channels based on negative control vector levels of hybridization.

Both p53 and p16 transcripts were considered absent (below levels of background hybridization) in all animals according to the Affymetrix software, but we were able to detect expression using the more sensitive method of RT-PCR.

Antigenomic probes are specifically designed by Affymetrix to provide an estimate of the non-specific background hybridization [ 32].

The images of all arrays were inspected for physical anomalies and for the presence of excessive background hybridization.

Note that, with the exception of YGR148c (missing spot of the putative cross-hybridizing gene YGL031c on our array) and YNL143c (hybridization signal of the putative cross-hybridizing gene YGR069w below the background signal), ratios at the cross-hybridizing spots are close to 1 or -1, as expected.

If we knew the background polyA hybridization Bi, for example, by inferring its value from hybridization with the oligo dT) probe, we could subtract the polyA background to obtain a corrected gene-specific hybridization signal, i.e. Hi' = Hi - Bi.

The addition of COT-1 DNA was performed to reduce background hybridization.

Background hybridization signal was determined using spike in negative controls, and all mRNAs had fewer than mean background+2 standard deviations were considered to be below the limits of detection.